PNC-27 (5mg)

(37 customer reviews)

$142.00

PNC-27 peptides are Synthesized and Lyophilized in the USA.

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Description

PNC-27 Peptide

PNC-27 is a synthetic peptide initially developed for its proposed inhibitive action on cancer cell proliferation. It is a member of the PNC family of probe proteins and is designed to attach to malformed cancer cells and induce cell necrosis, but bypass normal functioning cells. The PNC-27 peptide contains the HDM2 binding domain and transmembrane domain corresponding to residue 1226 on p53. Researchers suggest that it exhibits potential to bind to and kill cancer cells through membrane lysis or cell membrane destruction. Research studies have suggested that the PNC-27 peptide may be selective in targeting specific cancer cell types, prompting further research within the context of pancreatic cancer, breast cancer, leukemia, melanoma, and other cancer strains.[1]

The PNC protein was first developed in 2000. The PNC-27 peptide appears to be a non-toxic compound, attaching to and puncturing the membranes of individual cancer cells. As a result, rapid implosion may occur, leading to immediate cell death due to differences in osmotic pressure inside and outside the tumor cells. PNC-27 appears to have this potential through an affinity for binding to a protein called HDM2. Cancer cells typically display HDM2 in their cell membranes.[2] According to the researchers “PNC-27 targets HDM-2 in the membranes of cancer cells, allowing it to induce membranolysis of these cells selectively.” Immediately after exposure to the PNC-27 peptide, studies have observed that the peptide appears to move to HDM2. Binding to them creates pores or holes in the cell membrane, causing “membrane lysis” or death of the cell membrane. This process, in turn, may lead to the destruction of cancer cells. Research is ongoing.

Specifications

Molecular Formula: C188H293N53O44

Molecular Weight: 4031.7

Sequence: H-Pro-Pro-Leu-Ser-Gln-Glu-Thr-Phe-Ser-Asp-Leu-Trp-Lys-Leu-Leu-Lys-Lys-Trp-Lys-Met-Arg-Arg-Asn-Gln-Phe-Trp-Val-Lys-Val-Gln-Arg-Gly-OH

Synonyms: membrane residency peptide (MRP)

PNC-27 Peptide Research

PNC-27 and HDM2 Binding
In a paper published by the American Association for Cancer Research, Dr. Ehsan Sarafraz Yazdi et al. observed in-depth how the PNC-27 peptide appeared to exert its impacts, and what its apparent novel mechanism of action meant for cancer research in 2010.[3] The authors reported that the mechanism of action for PNC-27 may be due to the formation of oligomeric pores in the plasma membrane of tumor cells. Oligomeric pores are not formed in untransformed or non-tumor cells exposed to PNC-27, underscoring the peptide’s potentially selective toxicity towards cancer cells. In addition, the researchers pointed out that HDM2 as a target molecule has been observed to result in PNC-27 selectivity over cancer cells due to its mislocalization of the plasma membrane of cancer cells. The research proposes that the formation of a 1:1 complex between PNC-27, a peptide with potential anticancer properties, and HDM-2, a regulator of the p53 tumor suppressor, could be a crucial step in possibly triggering the formation of transmembrane pores. The study utilized a combination of theoretical modeling and experimental methods to investigate the interactions and structural foundations involved in this pore formation. Conformational energy analysis suggested that PNC-27 may form relatively stable complexes with HDM-2, potentially allowing the leader sequence to align in a way that minimizes interference with the core binding interaction, although its role in the overall pore structure cannot be definitively confirmed. Immuno-electron microscopy provided visual data of these complexes on the surface of cancer cells, where ring-like formations were observed at the putative pore locations.

Researchers found in a 2009 study that the three-dimensional structure of the p53 residue of PNC-27 might be directly superimposed on the design of the same residue bound to HDM2.[5] As a result, researchers may be able to exert PNC-27 to target HDM2 with the membrane of cancer cells. In particular, cancer cells appear to exhibit significant levels of HDM2 in their membranes, whereas non-transformed or non-cancerous cells do not appear to have substantial levels of HDM2. This process might allow the peptide to target cancer cells without selectively damaging the surrounding tissue.

In further experiments, transplantation of HDM2 containing a membrane localization signal into non-transformed cells that are less susceptible to PNC-27 has appeared to make these cells more sensitive to PNC-27. As a result, this provides further support to the hypothesis that the PNC-27 peptide may selectively attack HDM2 in the membrane of cancer cells, destroying them by membrane lysis while leaving functioning cells intact.

PNC-27 and Specific Cancer Cell Lines
PNC-27 might selectively target specific forms of leukemia cells and induce necrosis, particularly those displaying high levels of HDM-2 on their membranes. A research publication has concentrated on three acute myelogenous leukemia (AML) cell lines—U937, OCI-AML3, and HL60—that are genetically and phenotypically distinct.[6] These cell lines were verified to express elevated levels of membrane-bound HDM-2, which is hypothesized to be a critical factor for the possible cytotoxic action of PNC-27. The study suggested that PNC-27’s interaction with membrane-bound HDM-2 might be essential for its cytotoxic actions, as inferred from the colocalization of PNC-27 with HDM-2 observed through confocal microscopy.

In vitro experiments indicated that PNC-27 induced a reduction in the viability of the leukemia cells tested, implying that its cytotoxic actions might be selective for malignant cells, as normal rat mononuclear cells did not show similar vulnerability. The observed mode of cell death was necrosis, as indicated by the release of lactate dehydrogenase (LDH) from exposed leukemia cells, while apoptotic markers such as annexin V and caspase-3 activity were not increased. This observation suggests that PNC-27 likely induces necrosis through pore formation in the cell membrane, rather than triggering apoptosis. However, the study observed some variation in the potential of PNC-27 across different leukemia cell lines, which may be related to differences in HDM-2 isoform expression or other cellular factors that are not yet fully elucidated. This variability suggests that although PNC-27 may exhibit potential influence, its efficacy might not be consistent across all leukemia cell types.

A separate study examined the action of the PNC-27 on freshly isolated primary ovarian cancer cells.[7] These cells were specifically harvested from two ovarian cancer subtypes: mucinous cystadenocarcinoma and high-grade papillary serous carcinoma. In this study, PNC-27 appeared to exert an inhibitory influence on the proliferation of these primary ovarian cancer cells, alongside an apparent direct cytotoxic action. This cytotoxicity was inferred from the release of LDH, a biochemical marker associated with cell membrane disruption, suggesting that the peptide might compromise cellular integrity and induce cell death.

Disclaimer: The products mentioned are not intended for human or animal consumption. Research chemicals are intended solely for laboratory experimentation and/or in-vitro testing. Bodily introduction of any sort is strictly prohibited by law. All purchases are limited to licensed researchers and/or qualified professionals. All information shared in this article is for educational purposes only.

 

References

  1. Davitt K, Babcock BD, Fenelus M, Poon CK, Sarkar A, Trivigno V, Zolkind PA, Matthew SM, Grin’kina N, Orynbayeva Z, Shaikh MF, Adler V, Michl J, Sarafraz-Yazdi E, Pincus MR, Bowne WB. The anti-cancer peptide, PNC-27, induces tumor cell necrosis of a poorly differentiated non-solid tissue human leukemia cell line that depends on expression of HDM-2 in the plasma membrane of these cells. Ann Clin Lab Sci. 2014 Summer;44(3):241-8. PMID: 25117093.
  2. Sarafraz-Yazdi E, Bowne WB, Adler V, Sookraj KA, Wu V, Shteyler V, Patel H, Oxbury W, Brandt-Rauf P, Zenilman ME, Michl J, Pincus MR. Anticancer peptide PNC-27 adopts an HDM-2-binding conformation and kills cancer cells by binding to HDM-2 in their membranes. Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):1918-23. doi: 10.1073/pnas.0909364107. Epub 2010 Jan 11. PMID: 20080680; PMCID: PMC2836618.
  3. Sarafraz-Yazdi, Ehsan, et al. “MDM2 protein variants expression in the plasma membrane of cancer cells: A target for anticancer peptide PNC-27.” Cancer Research 70.8_Supplement (2010): 5770-5770.
  4. Aguon, Paul Muna, et al. “Experimental PNC-27 Therapy and Massive GI Hemorrhage: A Complication or Coincidence?: 1879.” Official journal of the American College of Gastroenterology| ACG 112 (2017): S1035-S1036.
  5. Sarafraz-Yazdi, E., Adler, V., Bowne, W., Sookraj, K., North, A., Niharny, P., … & Michl, J. (2009). Abstract# 884: Mechanism of action of PNC-27/-28 anti-cancer peptides. Cancer Research, 69(9_Supplement), 884-884
  6. Anusha Thadi et al, Targeting Membrane HDM-2 by PNC-27 Induces Necrosis in Leukemia Cells But Not in Normal Hematopoietic Cells, Anticancer Research 40 (9):4857-4867, September 2020 https://www.researchgate.net/publication/344117616
  7. Sarafraz-Yazdi E, Gorelick C, Wagreich AR, Salame G, Angert M, Gartman CH, Gupta V, Bowne WB, Lee YC, Abulafia O, Pincus MR, Michl J. Ex vivo Efficacy of Anti-Cancer Drug PNC-27 in the Treatment of Patient-Derived Epithelial Ovarian Cancer. Ann Clin Lab Sci. 2015 Fall;45(6):650-8. PMID: 26663795.
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This product is strictly for research/laboratory use only. Human or animal use and/or consumption is strictly prohibited by law. Only qualified and licensed professionals should handle these products. Any information found on Biotech Peptides is strictly for educational purposes only. Refer to our terms and conditions for more details.

Dr. Usman

Dr. Usman (BSc, MBBS, MaRCP) completed his studies in medicine at the Royal College of Physicians, London. He is an avid researcher with more than 30 publications in internationally recognized peer-reviewed journals. Dr. Usman has worked as a researcher and a medical consultant for reputable pharmaceutical companies such as Johnson & Johnson and Sanofi.

Additional information

Brand

Biotech Peptides

37 reviews for PNC-27 (5mg)

  1. Jade Hughes

    Personally I think the packaging could be better, my peptides have never broke or had any cracks but a more secure box could mean no breaks at all??

  2. Chris Wong

    It is always a quick and efficient procedure with them. My item arrived more quicker than expected, and the quality is excellent.

  3. Elija Stevenson

    They have high quality products, Like that it’s a US based company as well!

  4. tim wesson

    I struggled to find this product for so long and I think it is a bit pricey, but I think it’s worth it, since it can be so hard to find. It also works great in my research.

  5. kai chen

    Amazing company, very happy with the service I have been having for the past 7 months I have been with them.

  6. Kenneth Turner

    I’ve tried a couple other businesses before this one, and although the items were good, the customer service, which I believe to be a critical component of overall customer happiness, was lacking. I left them behind and came to you. Excellent customer service and kind staff.

  7. Chris Calvo

    My payment did not go through and was grateful that instead of going hours going back and forth through email the customer service representative called and let me know right away and we were able to solve it right there and then.

  8. Grayson woods

    All my products were dleivered when stated and nothing was missing.

  9. Bradley Mobeck

    You guys have definitely won me over

  10. Alvaro Villa

    I ran into an issue with payment. It didn’t go through and luckily their rep Taylor emailed me to let me know the situation. We were able to fix it quickly and I got my peptides in time.

  11. EJ McCoy

    Great stuff man.

  12. Greg Mathews

    Had some left over from my last project, if you follow the directions they have a great shelf life!

  13. Josh Espinoza

    Packaged great, even had the fragile sign on the box so it’s taken care of carefully.

  14. Brandon Effler

    After doing some research I decided to use Biotech as my peptide supplier. The peptides were great for what they cost.

  15. Tucker Steil

    Great service, staff really knows their stuff.

  16. Harry Bhangu

    My number one go to spot for peptides. Recently switched over to biotech as our lab orderes in bulk frequently and they seem to be the only one that can handle our needs.

  17. Kenny Hernandez

    BP always coming trough with the best products! Let’s go!

  18. Ken Shahryar

    They have a great variety of peptides, more than some other sites.

  19. Riley Kmiecik

    Great company overall. Ten out of ten

  20. Harees Sam

    You get better customer service here than other places

  21. Thomas Smith

    Peptides came in perfect condition, was a little worried with the heat since I’m on the other side of the country and how the travel/heat would effect the peptides but they were all good!

  22. Brandon Bellen

    Fantastic quality

  23. Steven King

    Products are good, better than most on the market

  24. Chance Moreno

    I am very happy with everything I’ve ordered from them up until now

  25. Jesse Alcala

    Danny was gracious and patient enough to deal with me and fix my payment issue. I had not chnaged my card and it expired. Danny called to let me know so we were able to get that fixed and was able to replace my order as it got canacled.

  26. Matt Goldberg

    Very easy and convienent check out process, had all my info stored already.

  27. Chris Massoud

    Jamie was able to help redo my purchase as I had to cancel the one I previously made. I had ordered the wrong peptides!

  28. Zahaan Jasani

    No issues with biotechs products. Quality has remained great and I have been purchasing from them for over a year now.

  29. Griffin Riggs

    Got a free bac water and I wasn’t even at the minimum for the buy!

  30. Cameron Mahlknecht

    Site was easy to maneuver and simple check out process. Got to give them props for the different payment options, not something you see everywhere. Made sure to get the standard shipment since it seems like they get products shipped out pretty fast anyway.

  31. Tony Provenzano

    Wish they had a way to let us know about restocks. Hate having to check in everyday for it.

  32. Jonovan Jeffery

    Biotech Peptides has great products! Been doing research with peptides for a long time and they are one of the best to work with.

  33. Jordan Powell

    Just great at service and getting the job done

  34. Bryan J

    Nice find! Lot of peptide options and surprisingly a lot of topical options as well.

  35. Ben Gugerty

    Definitely the best price-quality ratio out of all of the peptide suppliers I have come across so far. You’re getting a great product for a lot less.

  36. Conor Hurst

    I usually survey the internet for so long to find this product and once I did, I ordered it in batch. Everything came in perfect and I have no complaints at all

  37. Hank Landry

    Have been looking a lot on the internet for quality PNC and I have finally found it. If you’re considering buying this, it is well worth it

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